|
General
laboratory animal guidelines include:
- Too much blood collected at any one time
may cause hypovolemic shock, physiological stress and even death.
If smaller volumes are collected too frequently, anemia may
result.
- As a general rule, 10% of the total blood
volume can be collected at one time every 2-4 weeks. Or 1% at
intervals of 24 hours or more. Total blood volume can be calculated
as approximately 7.5% of body weight.
- The estimated volume at exsanguination is
approximately half of the total blood volume.
EXAMPLE
| Average Adult Weight |
Total Blood Volume |
Maximum Sample in mls q 2-4 Weeks |
Estimated Exsanguination Volume |
| 4 kg |
300 ml |
30 ml |
150 ml |
- Use aseptic technique.
- If you are not experienced in blood collection
techniques, training is
available.
| Collection Site |
Advantages |
Disadvantages |
| Jugular Vein |
- Large quantities of blood can be collected
- Anesthesia not required
|
- Requires specialized training
- Requires two people
|
| Cephalic Vein |
- Collection of small to moderate volumes
of blood
- Anesthesia not required
|
- Training recommended
- Requires two people
|
| Medial Saphenous Vein |
- Collection of small to moderate volumes
of blood
- Anesthesia not required
|
- Training recommended
- Requires two people
|
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