Adverse Effects:

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IACUC / LARC STANDARD PROCEDURES
IACUC / LARC STANDARD PROCEDURES

 

RETRO-ORBITAL BLEEDING OF RODENTS

Description of procedure:
This procedure is to be performed by or under close supervision of experienced personnel.
Animals must be fully anesthetized. Standard heparinized or non-heparinized micro-hematocrit capillary tubes can be used. The donor animal is held by the back of the neck and the loose skin of the head is tightened with thumb and middle finger to keep the animal stable. The tip of the capillary tube is placed at the medial canthus of the eye under the nictitating membrane.
A short thrust past the eyeball will enter the slightly resistant membrane of the sinus. The eyeball itself remains uninjured. As soon as the sinus is punctured, blood enters the tubing by capillary action. When the desired amount of blood is collected, the tube is withdrawn and slight pressure with a piece of gauze on the eyeball is used to prevent further bleeding.
For information regarding maximum blood collection volumes, please refer to: http://www.iacuc.ucsf.edu/Proc/awDosages.asp
Episodes of retro-orbital bleeding should be every other week only. Repeated bleedings should use alternate eyes.
Should you have any questions or would like training in the above technique, please contact the IACUC Training and Compliance staff by email at trainerIACUC@ucsf.edu or via phone at 476-2197.

Literature search words required:
Literature search was performed for refinement of this Standard Procedure on September 23, 2009:

Key Words Search Site Years Covered
Retro orbital blood collection, rodent
Pubmed
1966-2009
Retro orbital blood collection, rodent Google No Limit

Agents:

This procedure requires anesthesia. All agents administered to animals should be listed in the “Agents” section of RIO

Adverse Effects:

Adverse Effects
Procedure, Agent or Phenotype Potential Adverse Effects Management
Blood collection Injury to the eye leading to blindness, ocular ulcerations, puncture wounds, loss of vitreous humor, or keratitis Animals will be euthanized or a LARC veterinarian will be contacted.
Blood collection Infection Animals will be euthanized or a LARC veterinarian will be contacted.
Blood collection Excessive bleeding Animals will be euthanized or a LARC veterinarian will be contacted.

Monitoring Parameters:

Monitoring Parameters
Monitoring Parameters Frequency PI/Lab will Document
General appearance/activity Weekly No