IACUC / LARC STANDARD PROCEDURES
VASCULAR PERFUSION WITH FIXATIVES
FOR TISSUE COLLECTION
- This is a standard procedure for the vascular
perfusion of anesthetized animals with fixatives for preparing
tissues for histology. It is a terminal procedure.
- Animals should be anesthetized following
the Guidelines for Anesthetizing Animals for Research Purposes.
- The usual route of perfusion is transcardial.
Perfusion is usually done through the aorta or left ventricle.
The right atrium is cut open. Animals are transcardially perfused
through the ventricular catheter with saline followed by fixatives,
or are directly perfused with fixatives. The perfusion solution
drains through the incision in the atrium.
- Fixative solutions can also be infused through
other large arteries and drained from an incision in a vein.
- Fixatives are usually infused under gravity
or using a pump.
- The perfused animal is dead. Tissues are
collected for analysis.