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IACUC / LARC STANDARD PROCEDURES
RODENT IDENTIFICATION
All
these techniques are acceptable forms of identification. Individual
identification of animals plays a critical role in accurate record
keeping. There are several optional methods of identification
of rodents. Proper restraint plays an important role in most of
these techniques.
- Metal ear tags are inexpensive
and don't require anesthesia for application (although this
may facilitate proper placement) but the animal must be securely
restrained. The tags need to be appropriate size for the species
and should be applied to the distal 1/3 of the pinnae. If placed
correctly they generally last for at least 6 months. There is
a possibility of local infection and implantation sites should
be monitored occasionally.
- Ear notch punch is another
identification option. It is inexpensive and permanent, the
animal must be securely restrained but no anesthesia is required
if the animal is less than 3 weeks of age. Ear clipping remnants
can usually provide enough DNA for an initial PCR screening.
See below for code.
- Tail Tattoo is an acceptable
technique and offers a permanent means of identification. Disadvantages
include initial cost of equipment, some skill and experience
is needed and the need for anesthesia. Good for long term studies,
especially in rats.
- Electronic Transponders
are a recent option. Anesthesia is not required, but may facilitate
placement, as this only requires a subcutaneous injection. Initial
cost for reader is high but this is useful method for accurate
identification. Toe clipping is a traditional method of identification
and has the added benefit of high quality tissue sample for
analysis. However, the IACUC discourages toe clipping because
it has the potential to cause pain and distress and might alter
the gait, weight-bearing ability of a limb, and ability to feed.
- Toe clipping can be considered
under the following conditions: 1) Alternative methods of identification
must first be considered. 2) A written explanation of why it
is necessary is required, including a discussion of why alternate
methods are unsatisfactory. This will be considered and must
be approved by the IACUC. 3) It should only be performed when
mice are between 5-10 days of age and is limited to only one
digit per extremity. Mice should be anesthetized. Sharp sterile
scissors should be used and the foot should be cleaned with
a dilute betadine solution.
Ear notch punch code:
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