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IACUC / LARC STANDARD PROCEDURES
BROMODEOXYURIDINE (BRDU) TREATMENT OF MICE
(Posted November 2006)
Description of procedure:
Mice will be given BrdU in the drinking water (0.25-1
mg/ml) daily for up to 6 weeks or as daily i.p. injections (~2 mg in 100µl
of sterile saline) for up to 1 week. BrdU-containing water bottles are shielded
from light to prevent BrdU degradation and the water is replaced
twice per week. 1% glucose may be included in the drinking water during
the first week to overcome taste aversion. Total duration of the procedure
is 1 hr to 6 weeks, after which animals are euthanized.
Mice will be evaluated 3-4 times a week for the first
week, and weekly thereafter for general appearance, ability to
move normally around cage and reach food and water, ability to eat and drink,
grooming habits, skin color, lethargy, aggression (animals will be housed
individually if aggressive behavior is shown). Mice will be evaluated by
the Body Condition scoring index.
Mice with Body Condition score <2 will be euthanized.
Literature search words required:
In the section “Justifications and Alternatives” in
RIO you must include key words for potentially painful procedures. Literature
search was performed for refinement of this Standard Procedure on Sept
1, 2006:
| Key Words |
Search Site |
Years Covered |
| Bromodeoxyuridine (BrdU) |
Pubmed |
1958-2006 |
Agents:
- Bromodeoxyuridine (BrdU)
- All agents administered to animals should be listed in
the “Agents” section
of RIO.
Adverse Effects:
Adverse
Effects |
| Procedure,
Agent or Phenotype |
Potential
Adverse Effects |
Management |
| BrdU in drinking water |
Aversion/dehydration |
euthanasia |
| ip injections |
Infection from multiple injections |
euthanasia |
Monitoring Parameters |
| Monitoring Parameters |
Frequency |
PI/Lab will Document |
| Appearance/activity |
3-4 times week first week then Weekly |
No |
| Ability to move around cage/eat & drink |
3-4 times first week then Weekly |
No |
| Body condition score < 2 |
3-4 times first week then Weekly |
Yes |
| Describe
the conditions, complications, and criteria (e.g. uncontrolled
infection, loss of more than 15% body weight, etc.)
that would lead to removal of an animal from the study,
and describe how this will be accomplished (e.g. stopping
treatment, euthanasia). |
| Mice
with Body Condition score <2 will be euthanized. |
| For all investigators
housing animals with tumor formation, skin lesions, neurological
deficits, or that are in Category E, list the expected
characteristics/clinical presentations and endpoints of
the animal model and the criteria for euthanasia. Note:
The IACUC also requires such lists to be posted in the
respective animal rooms and monitored by the IACUC compliance
staff and LARC, to assure PI adherence to the endpoints
listed. |
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