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IACUC / LARC STANDARD PROCEDURES
MHC-MATCHED INTRAVENOUS ADOPTIVE CELL TRANSFERS
(Posted
November 2006)
Description of procedure:
Adoptive Transfer Protocol: A donor mouse is euthanized by standard procedures, and
lymphoid cells from donor mice are prepared as a single cell suspension
and adjusted to an appropriate volume of sterile PBS. Each mouse
will receive 0. 1- 0.4 ml of cell suspension. Cell numbers may vary between
1 x 10^4 and \1 x 10^8 cells. Recipient mice are injected i.v. (tail vein)
or anethetized and injected retro-oribitally using a small gauge (i.e. 30g ½)
needle. Mice
will be monitored continuously until fully recovered from anesthesia.
Mice will be evaluated for general appearance, ability to move normally
around cage and reach food and water, ability to eat and drink, grooming
habits, skin color, lethargy, aggression (animals will be housed individually
if aggressive behavior is shown). Mice will be evaluated by the Body Condition
scoring index. Mice will be monitored continuously after anesthesia until
ambulatory. Mice are then monitored 2-3 times for the first 5 days and weekly
thereafter.
Mice with Body Condition score <2 will be euthanized.
Literature search words required:
Literature search was performed for refinement of this Standard Procedure on
Sept 1, 2006
:
| Key Words |
Search Site |
Years Covered |
| Adoptive lymphocyte transfer |
Pubmed |
1963-2006 |
Agents:
- This procedure requires anesthetic for orbital injection. [specify whether analgesics
are required].
- All agents administered to animals should be listed
in the “Agents” section
of the protocol in RIO .
Adverse Effects:
Adverse
Effects |
| Procedure,
Agent or Phenotype |
Potential
Adverse Effects |
Management |
| retrooribital injections |
trauma to the eye |
euthanasia |
| Cell transfer |
Graft vs. host disease |
euthanasia |
Monitoring Parameters |
| Monitoring Parameters |
Frequency |
PI/Lab will Document |
| General appearance/activity |
2-3 times for 5 days then weekly
thereafter |
No |
| Ability to eat & drink |
2-3 times for 5 days then weekly
thereafter |
No |
| Weight loss/Body condition score < 2 |
2-3 times for 5 days then weekly
thereafter |
Yes |
| Describe
the conditions, complications, and criteria (e.g. uncontrolled
infection, loss of more than 15% body weight, etc.)
that would lead to removal of an animal from the study,
and describe how this will be accomplished (e.g. stopping
treatment, euthanasia). |
| Mice
with Body Condition score <2 will be euthanized. |
| For all investigators
housing animals with tumor formation, skin lesions, neurological
deficits, or that are in Category E, list the expected
characteristics/clinical presentations and endpoints of
the animal model and the criteria for euthanasia. Note:
The IACUC also requires such lists to be posted in the
respective animal rooms and monitored by the IACUC compliance
staff and LARC, to assure PI adherence to the endpoints
listed. |
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