ALTERNATIVES TO THE USE OF ANIMALS
ALTERNATIVES TO THE USE OF ASCITES MONOCLONAL
ANTIBODY PRODUCTION: UCSF CELL CULTURE FACILITY
In order to eliminate the use
of mice and to avoid pain, discomfort, or distress to animals
from the production of monoclonal antibodies using mouse ascites,
both OLAW and USDA encourage the use of alternative
methods to produce monoclonal antibodies in vitro, without compromising
the aims of the study.
Institutional Animal Care Use and Use Committees
are now expected to critically evaluate all protocols which propose
using the mouse ascites method, and only
allow in vivo production on the basis of strong scientific justification.
UCSF has responded to this by setting
up a core laboratory within the Cell
Culture Facility to produce
in vitro antibodies for UCSF investigators.
specific information on how to obtain in vitro antibodies,
please contact Sally Miramon, Technical Supervisor
of the Cell Culture Facility at 476-8830.
Additional Information regarding
antibody availability can be found on
The Antibody Resource Page.